[Histonet] Re:background staining with ultra view from Ventana

Gudrun Lang gu.lang <@t> gmx.at
Thu Mar 22 10:31:46 CDT 2007

The today's run was better than yesterday but background was still there. I
have cleaned the Benchy inside, but didn't change anything with the
Now I have swiched on the "ultra-wash" in the protocols. I have overlooked
this option and hope, that tomorrow I will have a cleaner result.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: eileen.lonergan <@t> verizon.net [mailto:eileen.lonergan <@t> verizon.net] 
Gesendet: Donnerstag, 22. März 2007 15:25
An: Joe Nocito; gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: Re: Re: [Histonet] background staining with ultra view from Ventana

Yes.  I just got in 2 new Benchmarks and started with the UltraView.  I had
Benchmarks before and used the iView with no background.  I am considering a
switchback to iView if I can't clear this up.   I am calling Ventana today
on this issue.  I thought I was crazy, so thanks for the posting.

If anyone else is experiencing this, please keep in touch.

Eileen Lonergan
Histopathology Manager
ConVerge Diagnostic Services
200 Corporate Place
Peabody, MA

elonergan <@t> convergedx.com

(978) 548-5206 

>From: Joe Nocito <jnocito <@t> satx.rr.com>
>Date: 2007/03/21 Wed PM 05:20:34 CDT
>To: gu.lang <@t> gmx.at, histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] background staining with ultra view from Ventana

>oh no, not again!! Last time I answered a question regarding this, I almost

>lost my job and was told to get off the Histonet. Nah, not worth it. I'll 
>keep my mouth shut this time.
>    See, you can teach an ole dog new tricks.
>----- Original Message ----- 
>From: "Gudrun Lang" <gu.lang <@t> gmx.at>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Wednesday, March 21, 2007 11:09 AM
>Subject: [Histonet] background staining with ultra view from Ventana
>> Hi to the Ventana users,
>> Have any of you experienced increased background staining with the
>> ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have
>> problems with this and I would like to know, if it is due to the reagens 
>> or
>> to the machine (Benchmark XT).
>> Thanks in advance
>> Gudrun Lang
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Eileen Lonergan
(207) 749-9617  cell
(207) 324-6468  home

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