[Histonet] sectioning spleen
stamptrain <@t> yahoo.com
Sun Mar 18 20:59:12 CDT 2007
Sounds like your tissue is overdehydrated or
overprocessed. Try reducing the time in alcohols and
maybe even in paraffin. Reducing alcohol times seems
to alleviate over 90% of the "too dry and crunchy"
problems we see.
--- mesruh turkekul <turkekul <@t> gmail.com> wrote:
> Dear Histoneters,
> I have tried manual paraffin embedding and embedding
> of spleen in the
> tissue processor.
> But I have noticed it is not easy to section. The
> tissue is so dry and
> crunchy. I really need to blow hard onto the block
> while sectioning to
> get nice sections. Do you have any tips in terms of
> sectioning. Or
> maybe fixation, processing?
> Mesruh Turkekul
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> Histonet <@t> lists.utsouthwestern.edu
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