[Histonet] sectioning spleen

Bartlett, Jeanine (CDC/CCID/NCZVED) jqb7 <@t> cdc.gov
Sun Mar 18 16:54:50 CDT 2007

Since spleen is so hemorrhagic I have found once the block is trimmed then prolonged soaking on a slushy, ice water bath prior to sectioning is a huge help.  If that is not enough, add some ammonium hydroxide to a separate container and fill with crushed ice and add your trimmed block.  Cover to help contain the fumes and this works wonders.
Jeanine Bartlett
CDc Atlanta

	-----Original Message----- 
	From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of mesruh turkekul 
	Sent: Sun 3/18/2007 4:44 PM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Subject: [Histonet] sectioning spleen

	Dear Histoneters,
	I have tried manual paraffin embedding and embedding of spleen in the
	tissue processor.
	But I have noticed it is not easy to section. The tissue is so dry and
	crunchy. I really need to blow hard onto the block while sectioning to
	get nice sections. Do you have any tips in terms of sectioning. Or
	maybe fixation, processing?
	Mesruh Turkekul
	Histonet mailing list
	Histonet <@t> lists.utsouthwestern.edu

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