[Histonet] does anyone know how to get better immersion fixation of mouse femur?

[Geoff McAuliffe]

Remove the other organs first (clamp off the blood supply) then perfuse.

Geoff

scoop <@t> mail.nih.gov wrote:

> Dear Histonetters,
>
> I have been using fixed mouse femur to do mouse bone marrows.  I get 
> much better fixation and preservation of bone marrow if I perfuse the 
> mouse than if I immersion fix the femurs.  However, I prefer not to 
> perfusion fix the mouse because perfusion fixation prevents me from 
> collecting other tissues unfixed.  Is there a way to improve the 
> fixation of the mouse femurs when I immersion fix?  I'm guessing that 
> the problem is I'm not getting good enough penetration - I suppose I 
> could poke a few holes in the femurs?
>
> Thanks in advance for your help,
> Sharon
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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