[Histonet] Nuclear artefact

[Gale, Nadia]

Urgent help required!!!
 
I know that I've seen previous messages regarding this artefact but need
some current views on the subject.
 
I am seeing nuclear artefact in some of our formalin fixed paraffin
embedded tissues.  The artefact appears as vacuoles or bubbles in the
nuclei with H&E.  I have tried multiple things to fix this to no avail.
 
Please, please provide your wisdom as to what could be causing this.  I
am at a loss!!!
 
 
Many thanks in advance,
Nadia Gale
Histotechnologist
Centre for Translational and Applied Genomics (CTAG)
3427-600 West 10th Avenue
Vancouver, BC V5Z 4E6
tel 604-877-6000 ext 2426
fax 604-877-6089
ngale <@t> bccancer.bc.ca