[Histonet] Cresyl Violet Staining

[Benjamin Cottam]

I have been staining rodent and moonkey retina fixed frozen sections
with cresyl violet, .5% for some time.  Recently the staining has been
washing out in the rinsing/dehyration steps.  I have tried inceasing the
time in the stain, increasing the stain concentration to 1%, and even
shortening the time in the wash and dehration.  I also tried moving
right from CV to 95% EtOH but after a few minutes, the staining has
washed out of the retina.  Any suggestions on what to do next would be
most helpful.
Benjamin Cottam
Sr. Research Assistant
OHSU-CEI