[Histonet] Paraffin specimen clamp

[AGrobe2555 <@t> aol.com]

"In this instance, I would embed the entire block as you would a piece of  
tissue, without melting the existing paraffin, and attach a cassette."
 
David,
I have tried this in the past with very little success.  The new, hot  
paraffin does not adhere to the old, cold paraffin enough to keep the blocks  
affixed to the cassette for any length of time when subjected to the shearing  
pressure of the blade while sectioning.  I have had to melt, and  re-embed the 
samples in order to section the samples.  The most  straight-forward way would be 
to find the appropriate clamp (hopefully at an  inexpensive price). 
 
Just curious, why can't you melt and re-embed?
 
Best of luck,
Albert   

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital





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