[Histonet] Paraffin specimen clamp

[Bonner, Janet]

David,
    We occasionally get some of these blocks, often from South America.  Though the paraffin is a different media, we melt them, let them soak/infiltrate for about two hours in our paraffin, and reembed them with a cassette. - But you cannot do that.  
     In this instance, I would embed the entire block as you would a piece of tissue, without melting the existing paraffin, and attach a cassette.   Chill, cut, and good luck! 
                                          -Janet

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Kinsley, David
Sent: Mon 6/25/2007 3:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Paraffin specimen clamp



Hi,

I cut paraffin sections on a Microm HM 355 microtome.  I recently
received some paraffin samples from Europe and they are rectangular
paraffin blocks measuring approximately 32x22x22mm without any plastic
cassette attached to them.  Does anyone know where I may be able to
purchase a different type of specimen holder/clamp that would hold the
paraffin block directly without the need for a cassette?

Unfortunately, I cannot melt the blocks and re-embed them with
cassettes. 

thanks in advance

Dave

 
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