[Histonet] picro sirius red

[Liz Chlipala]

Hello everyone
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We are running some picro sirius red for image analysis and were running =
into a bit of a problem.  We are staining rat heart, the collagen =
staining is quite nice but some of the muscle is staining a tad bit =
orangish rather than a really nice yellow.  Which is not a problem when =
you look under the microscope but once the images are captured for =
analysis thats were we run into problems.   We have tried making up =
fresh staining solution, etc.  differenciating more in the acetic acid =
but the muscle is stil a bit orange in color.  Any help would be =
appreciated. =20
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Thanks in advance
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Liz
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Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
HYPERLINK "mailto:liz <@t> premierlab.com"liz <@t> premierlab.com
HYPERLINK "http://www.premierlab.com/"www.premierlab.com
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Ship to Address:
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Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
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