[Histonet] RE: microglia in mouse brains

Leyva-Grado, Victor vic <@t> vetmed.wsu.edu
Wed Jun 20 15:33:23 CDT 2007


 
Hi Brett,

As Liz mentioned, F4/80 (a pan macrophage marker) will work. I've been
using Serotec's in frozen sections and it works fine for DAB staining.
Not very strong signal though when I used for fluorescence double
labeling. Some people also use anti-CD11b. I used it a couple times but
in my hands didn't work.

Victor H Leyva DVM
Department of VCAPP
Washington State University





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