[Histonet] Fixation for Her-2/neu -Where's the Beef???

[Rene J Buesa]

Before this regulation came to be we never left breast to fix for less than 12 hours and weekend specimens could be in NBF for up to 48 hours. There were even cases when we "re-sampled" the breast after several days in NBF and these "re-sampled" pieces gave the same results as the first time.
  Probably this CAP regulation is intended to standardize the procedure. I do not know of any peer reviewed articles recommending these fixation times (although I may be wrong as in other occasions).
  René J.



       
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