[Histonet] Perfusing Rat Lung

[Christine Tambasco]

   It's  been  a  few years since I did research, but we used to just use
   10%  NBF and we never had any complaints...you might want to give it a
   try.

   CHristine Tambasco, HT (ASCP)

   St. Marys Hospital @ Amsterdam, New York
       ______________________________________________________________

     From:  "Alexina Wempren" <AWempren <@t> skcc.org>
     To:  <histonet <@t> lists.utsouthwestern.edu>
     Subject:  [Histonet] Perfusing Rat Lung
     Date:  Tue, 5 Jun 2007 10:29:32 -0700
     >Does  anyone have a good protocol for perfusing rat lung?  We have
     been
     >flushing  with ringers, then 10% NBF, then ringers and ending with
     30%
     >sucrose  overnight but the morphology is not good. We add 0.8cc of
     air so
     >the  airways and vessels are not flat but it's still not great. We
     used
     >to  use  paraformaldehyde  but  my supervisor did not like it. Any
     help
     >would be greatly appreciated.
     >
     >    Thanks,           alexina
     >
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References

   1. http://g.msn.com/8HMBENUS/2755??PS=47575