[Histonet] Perfusing Rat Lung

[Greg Dobbin]

I have 2 references for you:

Microscopic changes induced by the intratracheal inoculation of amniotic fluid and meconium in the lung of neonatal rats. J. Martinez-Burnes, et al.  Histology and Histopathology (2002) 17:1067-76.

and

Ultrastructural changes in the lungs of neonatal rats intratracheally inoculated with meconium. J. Martinez et al.  Histology and Histopathology (2003) 18:1081-94.

If you prefer, I can fax you the Materials and Methods section(s) that describe the perfusion protocol that produced amazing results!
Cheers!
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385


>>> "Alexina Wempren" <AWempren <@t> skcc.org> 6/5/2007 2:29 PM >>>
Does anyone have a good protocol for perfusing rat lung?  We have been
flushing with ringers, then 10% NBF, then ringers and ending with 30%
sucrose overnight but the morphology is not good. We add 0.8cc of air so
the airways and vessels are not flat but it's still not great. We used
to use paraformaldehyde but my supervisor did not like it. Any help
would be greatly appreciated.

   Thanks,           alexina

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