[Histonet] Frozen sectioning of mouse mammary tissue

[Derek Papalegis]

Meg,
Drop the temp down to -30. That usually helps. Spray the blade and the 
anti-roll plate with cryo spray as well.

-Derek


mber <@t> vt.edu wrote:
> Hi all,
>    I have a grad student who needs help with frozen sectioning of mouse mammary
> tissue. He has not been able to cut any sections because it is fatty tissue. I
> haven't been able to watch him cut yet; I will be doing that tomorrow. In the
> meantime, if someone has suggestions and also a protocol, that would be very
> helpful. Thanks again.
> Meg Berger
> Histologist
> Animal and Poultry Sciences
> VA Tech
>
>
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-- 
Derek Papalegis HT (ASCP)
Histotechnician
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354