[Histonet] DAB residues removal
Olek Michalski
olek.michalski <@t> nencki.gov.pl
Tue Jun 5 06:48:36 CDT 2007
Dear Histonetters,
There are many procedures using DAB for staining. We apply some in our
lab, so sometimes there is need for cleaning Coplin jars covered w/ brown
residue. I normally do it by putting glassware into NaOH solution
containing some detergent over weekend and cleaing w/ brush afterwards.
Recently I heard there used to be more efficient protocol for DAB residue
removal but I can't find it. I know Dako provides DAB-away kit, but
supposedly it would be too much for couple jars per week. Do you know any
better way to deal with this problem?
Best regards
Olek Michalski
--
Laboratory of Neurobiology
of Development and Evolution
Nencki Institute of Experimental Biology
ul. Pasteura 3, 02-093 Warszawa, Poland
Tel. +48 22 5892268, Fax +48 22 8225342
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