[Histonet] DAB residues removal

Olek Michalski olek.michalski <@t> nencki.gov.pl
Tue Jun 5 06:48:36 CDT 2007


Dear Histonetters,

There are many procedures using DAB for staining. We apply some in our  
lab, so sometimes there is need for cleaning Coplin jars covered w/ brown  
residue. I normally do it by putting glassware into NaOH solution  
containing some detergent over weekend and cleaing w/ brush afterwards.  
Recently I heard there used to be more efficient protocol for DAB residue  
removal but I can't find it. I know Dako provides DAB-away kit, but  
supposedly it would be too much for couple jars per week. Do you know any  
better way to deal with this problem?

Best regards
Olek Michalski

-- 
         Laboratory of Neurobiology
        of Development and Evolution

  Nencki Institute of Experimental Biology
  ul. Pasteura 3, 02-093 Warszawa,  Poland
  Tel. +48 22 5892268,  Fax +48 22 8225342




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