[Histonet] Fat issues

[AGrobe2555 <@t> aol.com]

Hello All,
I have some vascular graft tissues that we have paraffin embedded and some  
of the tissues have extra-vascular fat.  The practice sample I  sectioned this 
morning seems to section well (other than the suture issues),  however the fat 
"blows up" as soon as I place the sections into the flotation  bath.  Is 
there some way to minimize this?  I will try cooling down  the water bath 
temperature, as it may have been a bit warm.
 
Any suggestions are welcome.
Thanks,
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital





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