[Histonet] Use of molecular sieve beads

[Constance McManus]

Ruth,
My husband -- an electron Microscopist -- uses them all the time for alcohols and other reagents.  I've used them before in histology and they really do a great job, but you need to allow time for them to work.  I would add mine on a Friday morning and by Monday or Tues of the next week the reagent (I was dehydrating alcohol, not xylene) was fine.  You need to use them in a certain ratio to the volume of reagent to be deydrated.  Also, there are different kinds of Molec seives used for different purposes.  I got mine from Sigma.  call them and they can help you out. 

Connie McManus, HT
University of Utah
School of Medicine
Dept. of Dermatology


----- Original Message ----
From: "Cazares, Ruth" <RCazares <@t> schosp.org>
To: histonet <@t> pathology.swmed.edu
Sent: Wednesday, July 25, 2007 8:49:03 AM
Subject: [Histonet] Use of molecular sieve beads


Hello histonetters,

I have just read an article about molecular sieve beads being used in
xylene to extract the water, and therefore extending the life of the
xylene indefinitely.  This article was given to me by a cytologist. She
came across it on the cytology equivalent to the Histonet. So my
question is, has anybody in histology used this method to keep xylene
moisture free? And if so, do it really extend the life of the xylene
indefinitely?

Any and all information is greatly appreciated!


Ruth Cazares, Histology Supervisor

Department of Pathology

Swedish Covenant Hospital

Chicago, IL 60625






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Thank you for your cooperation.

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