[Histonet] Re: Phosphorylation and IHC

[Gayle Callis]

Roche has PhosStop which should be added to the fixative and to all other 
buffers to prevent dephosphorylation in formalin fixed tissue sections for 
immunohistochemical staining.   They will send you a free sample. It is 
called PhosStop, a phosphatase inhibitor cocktail tablet.  Catalog # is 04 
906 845 001 for 10 tablets, and they have 20 tablet packages too.
www.roche-applied-science.com



  At 01:06 PM 7/17/2007, you wrote:

>Hi,
>I know phosphoylation  was kind of a hot topic at the last NSH.   I will 
>have to start looking at phosphoylation states in the near future.  Do you 
>have any suggestions?
>IHC on rat tisssues- spinal cord, brain and possibly dorsal root ganglia.
>
>Is perfusion or fixation preferred.
>What about frozen, how should the tissue be frozen.
>Any suggestion about buffers?

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610