[Histonet] c-fos staining

[Liz Chlipala]

Jaina

Why don't you make a notch or a small slice with a razor blade on one =
side of the brain.  Not too big that it would interfere with the =
interpretation.  We do this all the time with paraffin sections on rat =
brain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
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Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu =
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of =
jaina.negandhi <@t> sickkids.ca
Sent: Friday, July 13, 2007 8:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] c-fos staining

Hi,

I am carrying out c-fos staining on free-floating cross-sections of the =
brain. I am trying to map out the entire brain, and need to use a marker =
to distinguish between the left and right sides, as they are =
symmetrical.

Does anyone know of a marker (eg. dye) that I can use on a full brain =
sample without causing too much damage. I'd like to be able to mark it =
without gelatin embedding if possible.

Thanks,

Jaina Negandhi
Research Project Coordinator
Neuroscience and Mental Health
Hospital for Sick Children
Mc Master Building, RM 3006
Tel: 416-813-6551
Fax: 416-813-8724


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