[Histonet] re:Transferring tissue from OCT to NBF

Carl Hobbs carl.hobbs <@t> kcl.ac.uk
Fri Jul 13 13:41:32 CDT 2007

I personally prefer to allow the OCT-embedded frozen tissue to come to room 
temp( melt) before I rinse it in PBS and then place it in fixing fluid. ( 
eg: if it is a muscle bx, then I might  need to re-orient before 
fixing....hence a reason for thawing before fixing)
Depends on the tissue and it's pre-freezing orientation, imho.
If it is ovary and it has been optimally frozen ( no ice-crystal artefact) 
then just do what you suggested.
Caveat: One cannot guarantee that any results are of high fidelity unless 
you have an ovary that was fixed from fresh, as a positive control.

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