[Histonet] staining query
louise.renton <@t> gmail.com
Fri Jul 13 05:50:26 CDT 2007
Hi all, I have 2 questions to ask.
The first is...If I have counterstained my immuno too heaviliy with
haematoxylin, can I decolourise with acid alcohol & restain or will it
affect the DAB?
The second is.....Is there a tinctorial stain I can use to differentiate
multinucleated giant cells from muscle? I cannot use immuno, as the tissue
has been badly treated (poor things) and thus IHC is muddy & yucky.
Thank you, best reagrds & have a great weekend
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