[Histonet] staining query

[louise renton]

Hi all,  I have 2 questions to ask.

The first is...If I have counterstained my immuno too heaviliy with
haematoxylin, can I decolourise with acid alcohol & restain or will it
affect the DAB?

The second is.....Is there a  tinctorial stain I can use to differentiate
multinucleated giant cells from muscle? I cannot use immuno, as the tissue
has been badly treated (poor things) and thus IHC is muddy & yucky.

Thank you, best reagrds & have a great weekend






ent and only the moon howls".
George Carlin
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