[Histonet] predentine

[Geoff McAuliffe]

You could put Alcian Blue, Safranin O or Toluidine blue O in the 
fixative (yes, in the fixative) to improve retention of GAGs. There is 
no one stain that will demonstrate all connective tissue components.

Geoff

Rachel Neuenhoff wrote:
> I am trying to visualize the predentine layer in teeth. It is made up of a matrix of phosphoprotein and glycosaminoglycans (chondroitin-6-sulfate) and scattered type 1 collagen fibers. I've tried H&E with mixed results. Does someone know of stains that are specific to all three of these? I've read that alcain blue may work. Thanks!
>    
>   Rachel Neuenhoff
>   Texas A&M University at Galveston
>   rneuenhoff <@t> yahoo.com 
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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