[Histonet] Tracheal Whole Mounts

[Kim Merriam]

Hello everyone,

I have been asked to do some IHC staining on mouse tracheal whole mounts.  I was wondering if anyone has done this; I am looking for information on how to flatten out the trachea once it is removed from the animal.  

Do you think I should fix it first and then flatten it or fix and flatten at the same time.  Any thoughts or suggestions on how to flatten a mouse trachea would be greatly appreciated.

Thanks,
Kim
 
Kim Merriam, MA, HT(ASCP)
Cambridge, MA


 
____________________________________________________________________________________
8:00? 8:25? 8:40? Find a flick in no time 
with the Yahoo! Search movie showtime shortcut.
http://tools.search.yahoo.com/shortcuts/#news