[Histonet] Methods for flash freezing

Robyn Vazquez vazquezr <@t> ohsu.edu
Tue Jul 10 08:54:52 CDT 2007

Our department has purchased a LN2 tank and guns from a company called
Polar Cryogenics.  They may have other affiliates in Ohio.  Saves on a
lot of cost in the long run.
Surgical Dermatology
OHSU/Center for Health and Healing

>>> "Crystal Morris" <Crystalmo <@t> fmchealth.org> 7/10/2007 4:06 AM >>>

Good morning,
I am looking to get some info on flash freezing for frozen sections.
Currently we use the can freeze it spray, along with the heat extractor
in the cryostat, to do it faster, however I am concerned with safety and
feel that this poses a huge exposure risk. We have found that using the
heat extractor alone is not fast enough in cases with multiple specimens
and tend to get freeze artifact, most likely due to overzealous
pathologists. What is everyone else using? Do you use the canned spray?

Crystal Morris M.T.(ASCP)
Anatomical Supervisor
Fairfield Medical Center Laboratory
401 N. Ewing St.
Lancaster, Ohio 43130
(740) 687-8807

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