[Histonet] Freezing human skin with dry ice methanol?

[Jamie E Erickson]

Hi All,
            I have a question for anyone out there that is working with 
frozen tissue. I have a clinical lab that I am working with to obtain skin 
biopsies and they wants to freeze skin biopsies with dry-ice methanol 
instead of the dry-ice isopentane, Will this be a problem for me 
sectioning and doing IHC for cytokines and membrane bound antibodies? I do 
not like the idea of this but I don't  have any information on if it would 
be deleterious to freezing or do IHC this way. Anyone have any thoughts 
our suggestions?

Thanks

Jamie
_______________________________
Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com