[Histonet] lectin staining

[Rittman, Barry R]

Cherie
I would suspect  that the sugar moieties do.
The glycocalyx changes both during preparation for mitosis and also during all stages of differentiation for most cells.
Lots of useful infomation in "Lectins". by Sharon and Lis.
Will try to look for more specific information tomorrow as my copy of the book is at work.
Barry

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From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cherie Stayner
Sent: Sun 7/8/2007 9:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lectin staining



Hi all,

We are wanting to develop a lectin staining protocol using dolichos
biflorus (DBA) lectin to recognize kidney collecting duct cells in
culture (using immunofluorescence).  We have a biotinylated lectin
and a streptavidin conjugated fluorophore.  We are optimising our
procedure using IMCD cells but not all the cells are coming up
positive.  Does anyone know if the production of the sugar moieties
being recognized by a lectin vary depending on the stage of the cell
cycle (our cells are not synchronized), or do we just need to better
optimize our protocol?

Thanks,

Cherie
--
-------------------------------------------------------------
Cherie Stayner, PhD
Research Fellow
Developmental Genetics Group
Department of Pathology
University of Otago
Dunedin 9054
NEW ZEALAND

phone: +64 3 479 4129
fax: +64 3 479 7136
email: cherie.stayner <@t> stonebow.otago.ac.nz
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