[Histonet] lectin staining

[Cherie Stayner]

Hi all,

We are wanting to develop a lectin staining protocol using dolichos 
biflorus (DBA) lectin to recognize kidney collecting duct cells in 
culture (using immunofluorescence).  We have a biotinylated lectin 
and a streptavidin conjugated fluorophore.  We are optimising our 
procedure using IMCD cells but not all the cells are coming up 
positive.  Does anyone know if the production of the sugar moieties 
being recognized by a lectin vary depending on the stage of the cell 
cycle (our cells are not synchronized), or do we just need to better 
optimize our protocol?

Thanks,

Cherie
-- 
-------------------------------------------------------------
Cherie Stayner, PhD
Research Fellow
Developmental Genetics Group
Department of Pathology
University of Otago
Dunedin 9054
NEW ZEALAND

phone: +64 3 479 4129
fax: +64 3 479 7136
email: cherie.stayner <@t> stonebow.otago.ac.nz
-------------------------------------------------------------