[Histonet] marker to measure if sorted or FACSed cells are
contaminated?
scoop <@t> mail.nih.gov
scoop <@t> mail.nih.gov
Fri Jul 6 15:18:57 CDT 2007
Dear Histonetters,
I'm sorting mouse bone marrow cells (using magnetic beads). I would
like to do a western on my sorted cells to determine whether the
erythroid cells are contaminated with macrophages or B-cells. Does
anyone know of an antibody to a mouse bone marrow macrophage marker
and a mouse bone marrow B-cell marker that I can use for this
purpose? I was thinking about F4/80 and CD11 but I don't know if
they work in a western.
Thanks in advance!
Sharon
More information about the Histonet
mailing list