[Histonet] A question on bone sections

[Molinari, Betsy]

As long as the bone is decalcified properly you should have no problem
cutting the tibia on a regular microtome with a disposable blade. I cut
mouse tibia this way and have no problems.

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yu, Jian
Sent: Thursday, January 25, 2007 9:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] A question on bone sections

Dear all,

We have parrafin embedded bone of mouse tibia (decalcified).  Can
sections (longitudinal) of the bone be cut with a regular microtome
blade or a special blade? We intend to do H&E staining on these sections
and cut soft tissue sections (mouse) in our own lab routinely.  

This is the first time we are dealing with bone.  Any advice will be
very helpful and appreciated.

Thanks, Jian
*******************************************************************
Jian Yu, Ph.D.
University of Pittsburgh Cancer Institute
Phone: 412-623-7786
Email: yuj2 <@t> upmc.edu
*******************************************************************


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