[Histonet] A question on bone sections

DiCarlo, Margaret MDiCarlo <@t> KaleidaHealth.Org
Thu Jan 25 09:50:06 CST 2007


Jian,

Yes, you can cut the decaled mice tibia with a disposable or regular
microtome blade.  Before cutting, I soak a few of the blocks in decal,
in an empty slide box.  Set it on top of a block of ice and add some
water, soak for about 10 minutes, rinse block under cold water and then
it is ready to be cut.

Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital 
Buffalo, NY  14203
716-859-1293


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yu, Jian
Sent: Thursday, January 25, 2007 10:06
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] A question on bone sections

Dear all,

We have parrafin embedded bone of mouse tibia (decalcified).  Can
sections (longitudinal) of the bone be cut with a regular microtome
blade or a special blade? We intend to do H&E staining on these sections
and cut soft tissue sections (mouse) in our own lab routinely.  

This is the first time we are dealing with bone.  Any advice will be
very helpful and appreciated.

Thanks, Jian
*******************************************************************
Jian Yu, Ph.D.
University of Pittsburgh Cancer Institute
Phone: 412-623-7786
Email: yuj2 <@t> upmc.edu
*******************************************************************


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually.

Check us out at: www.WesternNYshospitalofchoice.org

CONFIDENTIALITY NOTICE: 
This email transmission and any documents, files, 
or previous e-mail messages attached to it are 
confidential and intended solely for the use of the 
individual or entity to whom they are addressed. 
If you are not the intended recipient, or a person 
responsible for delivering it to the intended recipient, 
you are hereby notified that any further review, 
disclosure, copying, dissemination, distribution, or 
use of any of the information contained in or attached 
to this e-mail transmission is strictly prohibited. 
If you have received this message in error, please 
notify the sender immediately by e-mail, discard 
any paper copies, and delete all electronic files 
of the message. If you are unable to contact the 
sender or you are not sure as to whether you 
are the intended recipient, please e-mail 
ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.



More information about the Histonet mailing list