PMonfils <@t> Lifespan.org
Thu Jan 25 09:49:18 CST 2007
-30 is too cold to section testicles. I cut them at -15 C. Sometimes even -12 C.
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Inga Hansson
> Sent: Thursday, January 25, 2007 4:54 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] testicles
> Hi everyone,
> A collegue of mine is trying to section frozen rat testicle (perfused
> and cryoprotected in sucrose) at -30°C. Is that a good temperature to
> use? The sections seem fragile and tend to break. They´re 10 microns
> thick. Or is it bad fixation?
> Thanks in advance for any help!
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