[Histonet] embedding without a station?

Anila Syed syedab <@t> totalise.co.uk
Wed Jan 24 12:12:16 CST 2007

Wow!! Thanks for all the suggestions and trips down memory lane! I dont get
how paper could have been used as moulds!! and I love the teapot idea!! As
for the asbestos and lead etc, I won't mention any of that to our safety
officer :)

Thanks everyone, I will try to go with a beaker in the incubator and a
hotplate, but will also try the doughnuts approach :)


----- Original Message ----- 
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, January 24, 2007 4:50 PM
Subject: RE: [Histonet] embedding without a station?

We didn't have embedding stations when I started in histology. We had three
stainless steel pitchers of melted paraffin, maybe 500 ml each, in the
paraffin oven. We worked on a large, low temperature hotplate, called a
"slide warmer".  We would take one of the pitchers to the "work station",
place it on the slide warmer.  We would take the last paraffin container,
containing the processed specimens, off the rotary processor and plug it in
next to the hotplate. Take an embedding mold (these were made of paper,
folded by the histotechs at the end of the previous workday), write the
specimen ID on it, place it on the hotplate, pour paraffin into it from the
pitcher, unwrap the tissue sample (which were processed wrapped in lens
paper - no such thing as cassettes), place it in the mold, then transfer the
mold to a metal plate on top of a cakepan full of ice.  When the pitcher of
paraffin started to get too cool, replace it in the oven and take another
pitcher. Sounds pretty primitive - mainly because it was - but it got the
job done.

> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Anila Syed
> Sent: Wednesday, January 24, 2007 5:05 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] embedding without a station?
> Dear All,
> I have hundreds of carotid plaques to embedd. I have a tissue processor,
> no embedding station. Would anyone attempt to do this without an embedding
> station or do you think I should go and try to find the facilities
> somewhere?
> What did people do before embedding stations?
> Many thanks for your input and opinions,
> Anila Syed
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

No virus found in this incoming message.
Checked by AVG Free Edition.
Version: 7.1.410 / Virus Database: 268.17.8/649 - Release Date: 1/23/07

More information about the Histonet mailing list