AW: [Histonet] Goldner's Trichrome Staining

[Gudrun Lang]

Your protocol sounds like an onestep trichrome, but in my literature Goldner
consists of more.

nuclei-staining with weigert
Staining in a mixture of acid fuchsin and Ponceau de Xylidin 
(0,2 g Ponceau de Xylidin and 0,1 g Acid fuchsin in 300 ml Aqua dest. Plus
0,6 ml 100% acetic acid)
Rinse in 1% acetic acid
Differentiate in PMA-Orange G solution (few minutes)
(3-5g Phosphormolybdic acid and 2 g Orange G in 100 ml Aqua dest)
Rinse in 1% acetic acid
Counterstain with lightgreen (0,1% 100 ml plus 0,2 ml 100% acetic acid)
5 min 1% acetic acid
Dehydrate, clear and mount



Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Tiffany
Pitts
Gesendet: Mittwoch, 24. Jänner 2007 17:29
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Goldner's Trichrome Staining

Hello and Good Morning all!

I hoped not to have to bother you all with this question but an extensive 
search through the Histonet archives did not turn up the information that I 
needed so here I am asking for help. My situation is as follows:

I have been cutting MMA embedded mouse bones and staining them with a 
version of Goldner's trichrome stain. I learned this technique from a 
colleague who has since moved far far away and is no longer in the sciences.

I have been merrily chuffing away on my project when I came to a screeching 
halt two days ago because I have run out of one of the staining reagents. I 
thought to just look it up on Stains File and get back to business but every

protocol I have found so far differs wildly from the protocol I have been 
following (that has been giving me pretty satisfactory results) In a nut 
shell here's what I've been doing:

Deplasticize
rehydrate
wash
Weigart's Hematoxilyn
wash
1% HCl
wash
Saturated Lithium carbide Sol'n
wash
"Goldner's Trichrome Stain" reagent (?!?!?)
wash for 1 min
dehydrate and coverslip

It is the "Goldner's Trichrome Stain" reagent that I have run out of and 
when I looked up my former colleague's notes she has "See Julie" instead of 
a recipe. Well, Julie is no longer with us either and I am running out of 
places to look! Can anybody tell me what they think this All-in-one reagent 
might be? Or perhaps suggest a different solution (pun not intended)? I 
would be so grateful.
Thank you so much,

Tiffany Pitts
Department of Urology
University of Washington
Seattle, WA


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