[Histonet] embedding without a station?

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Wed Jan 24 09:35:32 CST 2007


It wasn't that long ago - -1989, when I first started working in Honolulu 
- the contract lab we worked with used ceramic molds with little tags of 
paper to ID the blocks.
They used the now-common plastic cassettes to process, but then threw them 
away when they embedded.   The thinking was that the ceramic molds used 
less paraffin.   What a quality control nightmare that was. 
Jackie O'



"Ford Royer" <froyer <@t> bitstream.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/24/2007 09:24 AM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] embedding without a station?






Doesn't any body remember good 'ole lead "L's"?

Stainless steel griffon beaker in the oven worked pretty good, but we 
where
in hog heaven with they broke down and got us a "heated paraffin beaker".
The beaker itself wasn't actually heated.  There was an electrically 
heated
sleeve that the beaker sat in on the bench that kept the paraffin 
liquefied.
I think that it was on of Dr. McCormick's ingenious inventions...

How did we EVER survive? ... Open containers of Xylene, Formalin, Asbestos
gloves and pads, Lead "L's", and cigarette ash trays located ant each
station.

~ Ford

Ford M. Royer, MT(ASCP)
Minnesota Medical, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
CELL:  612-839-1046
Phone:  763-542-8725
Fax:  763-546-4830
eMail:  clinicallab <@t> minnesotamedical.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith, 
Allen
Sent: Wednesday, January 24, 2007 8:59 AM
To: Anila Syed
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] embedding without a station?

I have never felt that embedding stations are worth the counter space they
take up.  I just barely melt my Paraplast in a Pyrex beaker on a hotplate
and maintain its temperature in a 60 degree oven. I set out the mold on 
the
counter and pour in enough Paraplast to quarter fill it.  Since the beaker
is hot, I keep a cotton glove on my left hand.  I leave my right hand bare
to handle the forceps.)  I warm my forceps in the flame of an alcohol 
lamp,
and position my tissue on the bottom of the mold, set an embedding ring in
place, and fill the mold. I wipe the lip of the beaker with a paper towel
(usually one that has already been used for drying my hands). Everything
except the oven can be put away in a cupboard, leaving me with counter 
space
for other projects.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anila Syed
Sent: Wednesday, January 24, 2007 8:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] embedding without a station?

Dear All,

I have hundreds of carotid plaques to embedd. I have a tissue processor, 
but

no embedding station. Would anyone attempt to do this without an embedding 

station or do you think I should go and try to find the facilities 
somewhere?

What did people do before embedding stations?

Many thanks for your input and opinions,

Anila Syed 


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