Fwd: Re: [Histonet] HER2 and the 48 hour rule

Tue Jan 23 14:30:34 CST 2007

Hi Rene,
I remember you mentioning the mineral Oil  in the past. Could you elaborate 
on the quality of the tissues. I would love to get rid of Xylene.
Thanks

Sheila Adey HT MLT
Port Huron Hospital
Michigan

>From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: Fwd: Re: [Histonet] HER2 and the 48 hour rule
>Date: Tue, 23 Jan 2007 11:48:29 -0800 (PST)
>
>I meant MINERAL OIL.
>   René J.
>
>Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>   Date: Tue, 23 Jan 2007 11:43:24 -0800 (PST)
>From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>To: sheila adey <sheila_adey <@t> hotmail.com>, ploykasek <@t> phenopath.com,
>1dpeterson <@t> meriter.com, histonet <@t> pathology.swmed.edu
>CC:
>Subject: Re: [Histonet] HER2 and the 48 hour rule
>
>Sheila:
>I would never leave tissues in xylene, it makes them very brittle and hard 
>to section. This is one of the reasons why I stopped using xylene 
>altogether in 1998 and substituted it with mineral (where you can leave the 
>tissues for as long as you want).
>René J.
>
>sheila adey wrote:
>This prompts a new question.
>I'm curious as to whether other labs that don't work weekends leave their
>tissues in formalin with a delayed start or do you start the processor 
>right
>away and leave the tissues in Xylene as stated below?
>
>
>
>Sheila Adey HT MLT
>Port Huron Hospital
>Michigan
>
>
>
>
>
> >From: Patti Loykasek
>
> >To: "Peterson, Dan" <1dpeterson <@t> meriter.com>,histonet
> >
> >Subject: Re: [Histonet] HER2 and the 48 hour rule
> >Date: Tue, 23 Jan 2007 10:06:08 -0800
> >
> >Dear Dan,
> >Welcome to the new world of Her2 testing! There will definitely be 
>changes
> >in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge 
>all
> >techs to get a copy of the guidelines and read them.
> >That being said, the time in fixation is important for Her2 testing. With
> >heat retrieval I think that too little fixation is worse than >48 hours,
> >but
> >for now we must comply with 6-48 hours. I was taught (a long time ago) 
>that
> >it is best practice for the weekend schedule fixation to reflect the rest
> >of
> >the week, and that it is preferable to leave the tissue longer in xylene.
> >That xylene would have less bad effects than longer time in formalin. A
> >longer time in alcohol would be deleterious to many IHC stains, and not
> >recommended for Her2.
> >Whatever schedule changes you decide to implement, hopefully you can test
> >your new schedule on some tissue before full implementation of a new
> >processing schedule.
> >
> >
> >Patti Loykasek BS, HTL, QIHC
> >PhenoPath Laboratories
> >Seattle, WA
> >
> >
> >
> >
> >
> >
> > > Hello Histonetters!
> > > Maybe this has been discussed before, and if it has, I apologize in
> > > advance, but I need a little input. Now that the CAP has mandated a 6
> > > -48 hour window of fixation time for specimens that may have Her-2 neu
> > > performed, what are you doing about weekend specimens? We JUST (after
> > > 25+ years) got rid of the need for techs to come in on Saturdays, and
> > > would like to be able to continue this trend. However if a breast bx 
>is
> > > done at an outside account on a Thursday afternoon, and does not get
> > > grossed by our staff until Friday, right now a our processors are set 
>to
> > > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So
> > > here are my questions:
> > > Do you set up the processor to sit in 70% OH after the formalin fix? 
>Do
> > > you let it sit in the paraffin from Saturday until Monday? (Is heat 
>too
> > > prolonged?)
> > > Or do I have to break the news to staff that if their name is up, and 
>a
> > > breast bx comes in, they're coming in Saturday am?
> > > We do not have a microwave processor (yet), but soon.
> > > Any and all responses will be greatly appreciated!
> > >
> > > Daniel R Peterson HT(ASCP)
> > > Histopathology Section Head
> > > Meriter Laboratories
> > > (608)-267-6557
> > > 1dpeterson <@t> meriter.com
> > >
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