[Histonet] cryo-embedding and other "cool" stuff. :)
debbiekeith <@t> cox.net
Mon Jan 22 17:27:04 CST 2007
for those that helped me with my cryo-issues AND those that were
amused/tickled/glad it wasn't them....I realized there was another
installation of the "cryostat debacle of 2006".
so many people helped me during the "crisis"... and a couple worth
Barry (technician) of BelAir, Don (repair technician) from Leica and Jan
Minshew (HTL) with Leica. THANK YOU GUYS!!! :)
after replacing the parts i'd gotten from BelAir and doing everything Jan
suggested, i felt like i sorta figured it all out. the machine still had
some odd behaviors, or pecidillos, if you will. overall, i felt i could
work around them. maybe even embrace them the way you would the behaviors
of your own teenager. begrudginly.... but you'd still hug it when it was
well, after our newfound compatibility.... the ex-sailor was scheduled to
work. what could he do in ONE day?!
funny you should ask. :)
he tightened the screw on top so HARD... that he stripped the threads
COMPLETELY out and you could remove the lever but simply lifting it
i called the repair folks and they said it would be WEEKS to get the part
in. so, i asked "can't i just heli-coil it?" the repair guy was VERY
skeptical. he sounded like he didn't even WANT to know what i was up
to. (smart man!)
i took it home and my hubby fixed it with parts we had in the garage!! (we
have a few british bikes in the garage, so there are metric bits/parts all
over!) the cryostat was not only GOOD as new... but BETTER! the original
part is aluminum.... and the heli-coil is steel... so it is stronger,
harder and FASTER. it is the Steve Austin of cryostats.
before the holidays i'd been sorta looking at cryo-embedding systems. the
ones out there are great... but they aren't really Mohs-Friendly. know
what i mean? so i MADE my own. i can't BELIEVE how awesome it IS!!! i
did exhaustive research on metals/heat syncs/thermal conductivity... and
created a system that reduced my processing time by 50%. my
turn--around-time is about 10 minutes from fresh tissue to slides on the
scope. (i hand stain!) is it too good to be true....? nope. every day,
when i clean the inside of the cryostat... i lovingly caress the chuck
holder... and blow a kiss at the cryo-embedding system. (utilizing
universal precautions the whole while! natch!)
so, now... my slides are purty, my turn-around-time is shorter and my
cryostat is making "THE noise". it IS a happy new year, indeed.
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