[Histonet] Markers of oxidative damage

[bhewlett <@t> cogeco.ca]

> Hi Sony,

8-OHdG and 8-OG can be demonstrated by means of their biotin-like reactivity.
We have seen this in a number of cases when it appeared as a specific background, in both the patient test and negative
reagent control when staining for assorted antigens by IHC.

See following reference;
Struthers L, Patel R, Clark J, Thomas S.
Direct detection of 8-oxodeoxyguanosine and 8-oxoguanine
by avidin and its analogues.
Analytical Biochemistry 1998; 255: 20-31.

Regards,

bryan


> Hi all,
>
> I am trying to get information on markers of oxidative damage (8-OHdG, 4-HNE
> or any others that might work). I have some formalin fixed paraffin embedded
> mouse kidneys and I need to assess the oxidative damage to the tissue via
> immunohistochemistry.
> If anyone has used such markers and has any protocols or suggestions I would
> highly appreciate it.
>
> Many thanks,
> Sony Prasad.
> Research Student,
> University of Sheffield.
>
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