[Histonet] Ventana IHC Staining

Zummak Melissa Melissa.Zummak <@t> ssfhs.org
Fri Jan 19 07:05:23 CST 2007


I have experienced that problem also and the explanation that was given
to us by Ventana was that we must have had an air bubble in the
dispenser and unfortunately there is no way for the instrument to sense
that there is an air bubble present or that the instrument did not
dispense any reagent onto the slide. We always check the dispenser to
see if we can see any air bubbles before we use it but that has not
always worked.

Melissa Zummak
AP Supervisor
Alverno Clinical Labs 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Chaussey, Leslie
Sent: Thursday, January 18, 2007 12:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana IHC Staining

We're noticing some issues with our IHC staining.  It's very random but
we've been seeing instances where the positive control tissue stains
fine but the patient tissue doesn't stain at all.  In one instance, we
re-ran the stain manually & it worked fine.  We generally place patient
tissue on the same slide as the control tissue using the red box control
slides (red box is painted on the back of the slide). We have some ideas
but wanted to check in to see if anyone else using Ventana
Instrumentation (Benchmark XT) has seen that problem as well.  Thanks
for any feedback - it's greatly appreciated.    


Leslie J Chaussey


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