[Histonet] project on microwave
ree3 <@t> leicester.ac.uk
Fri Jan 19 03:27:13 CST 2007
Well a vendor would say that!.
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Phil
Sent: 18 January 2007 20:54
To: rosadel holgado
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] project on microwave
A microwave vendor weighs in:
EBS' position is that domestic microwaves not be used for lab work, but
then, we're in the position of manufacturing lab microwaves. :-) That
said, in this case I can understand the temptation to do so, given the
academic context and undoubtedly inherent budget constraints. I would be
careful, however: of all the operations lab microwaves can be used for,
fixation and tissue processing are the most demanding and require the
tightest temperature control.
Besides the issue of a temperature probe (most domestic microwaves don't
have one) there is the issue of magnetron cycle time; domestic
microwaves' cycle times are typically too long to allow the temperature
control required for small, delicate histological samples. The chances
of cooking your tissue are great.
I'd explore other options: local locations with laboratory microwaves
who might let you into their lab. Especially for fixation and
processing, a true laboratory microwave is the surest path to success.
Microwave Product Manager
Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026
Tel: 800.992.9037 x 341
pmcardle <@t> ebsciences.com
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rosadel holgado wrote:
> I am a newbie here and I wonder if it's appropriate to be asking
questions here about my project. I am planning to do a project for my
MSc soon and I was thinking of doing something about microwave fixation
and microwave assisted processing. Does anyone know of a protocol I can
cite for using a domestic microwave for the fixation?
> Rosadel Salita
> Surrey, England
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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