[Histonet] Vital stain for mouse v. human cells

[Liz Chlipala]

Jackie

I have not heard of a vital stain, but I know that this can be determined
via in-situ hybridization.  You can stain for mouse satellite DNA repeat
sequences, you need to get an oligo probe that is specific to the mouse
genome and will not detect human.  We have one that we have used for mouse
verses porcine tissue.  We get our probes from Genedetect.  I also have a
few papers on this if you would like them.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
 
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University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie M
O'Connor
Sent: Thursday, January 18, 2007 2:29 PM
To: Histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] Vital stain for mouse v. human cells

Earlier today I think I read a post from someone about staining only mouse 
cells in a human xenograft, and of course, I deleted it instead of saving 
it.
Would someone please give me the general gist of how someone is staining 
only mouse cells?  I thought it was a vital stain of some sort.
thanks

Jackie O'
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