[Histonet] Ventana IHC Staining

[Douglas D Deltour]

A number of things can cause inconsistent staining. Here are a few. 


Barcode label properties Altered
1) The hydrophobic properties of the labels can cause nonstaining of the
issue if it is placed to close to the label.
2) Placement of the labels on the slides-Crooked labels can cause the
solutions to wick off the edge of the slides.
3) Cutting the Labels edges-If the labels are cut it increases the slides
surface area and can cause light staining.
4) Aging-The glue can get old

Silanization of Glass Slides
1) Making of a charged glass slide. Different Vendors have different
quality.
2) Consider factors that compromise the adhesion:
A. "Double dipping of slides". This is where the control is picked up first,
then at a later time the patient is picked up and the slide is wet twice.
B. "Stay-On" used with plus slides.
3) Consider the factors that produce unwanted background.
A. Gelatin in the waterbath.
B. Elmer's Glue in the waterbath.
4) Check the storage conditions, age and lot numbers of the slides.

If these steps don't help then do a Vortex Mix test. 





 
Douglas D. Deltour HT(ASCP)
Histology Supervisor
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chaussey,
Leslie
Sent: Thursday, January 18, 2007 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana IHC Staining

We're noticing some issues with our IHC staining.  It's very random but
we've been seeing instances where the positive control tissue stains
fine but the patient tissue doesn't stain at all.  In one instance, we
re-ran the stain manually & it worked fine.  We generally place patient
tissue on the same slide as the control tissue using the red box control
slides (red box is painted on the back of the slide). We have some ideas
but wanted to check in to see if anyone else using Ventana
Instrumentation (Benchmark XT) has seen that problem as well.  Thanks
for any feedback - it's greatly appreciated.    

 

Leslie J Chaussey

 




-----------------------------------------
This message and any included attachments are intended only for the
addressee. The information contained in this message is
confidential and may constitute proprietary or non-public
information under international, federal, or state laws.
Unauthorized forwarding, printing, copying, distribution, or use of
such information is strictly prohibited and may be unlawful. If you
are not the addressee, please promptly delete this message and
notify the sender of the delivery error by e-mail.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet