[Histonet] Ventana IHC Staining

Janice Mahoney jmahoney <@t> alegent.org
Thu Jan 18 13:12:06 CST 2007

Oh Boy, here we go again.
I'm a Ventana user and when we have had this occasional problem it has been something simple like needing to do the routine maintenance.  Have you performed your function tests, checked your heat pads and decontaminated your instrument?  
I also have found the Ventana customer service people helpful.
Hope this helps you solve your problem.

>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 01/18/2007 12:46 PM >>>
  I suggest you to go to Histonet Archieves and find out about this very same recurrent problem with the Ventana Benchmark (and a very large and controversial thread about the likes and dislikes about Ventana).
  I personally do not like closed systems for IHC, I always used and preferred Dako and cannot help you on this problem. Check the Archieves!
  René J.

"Chaussey, Leslie" <Lchausse <@t> nmh.org> wrote:
  We're noticing some issues with our IHC staining. It's very random but
we've been seeing instances where the positive control tissue stains
fine but the patient tissue doesn't stain at all. In one instance, we
re-ran the stain manually & it worked fine. We generally place patient
tissue on the same slide as the control tissue using the red box control
slides (red box is painted on the back of the slide). We have some ideas
but wanted to check in to see if anyone else using Ventana
Instrumentation (Benchmark XT) has seen that problem as well. Thanks
for any feedback - it's greatly appreciated. 

Leslie J Chaussey

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