[Histonet] Trying to get consensus - Frozen section immediate postfixation or air dry

[Terri Braud]

One person's opinion
It depends on the application for me, but generally, if morphology is important, then I fix them right away.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supv.
Laboratory, Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3689

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of John
MacDougall
Sent: Thursday, January 18, 2007 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Trying to get consensus - Frozen section immediate
postfixation or air dry


Hi Histonet

I have seen two basic camps regarding the treatment of frozen sections
once the section hits the slide - One camp fixes immediately and one
camp leaves the section to air dry, sometimes overnight.  I realize that
there is likely not a "right" answer, but are there advantages to one
process method over another? I'm in the midst of trying out both
processes and evaluating myself, but in the meantime I'd like to hear
feedback from the community.

 

Thanks

 

John 

 

John MacDougall, Ph.D.

Lead Senior Scientist

Infinity Pharmaceuticals

780 Memorial Drive

Cambridge, MA

02139

 

Voice - 617.453.1214

Fax - 617.453.1001

 



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