[Histonet] Trying to get consensus - Frozen section
immediate post fixation or air dry
gcallis <@t> montana.edu
Thu Jan 18 12:21:37 CST 2007
How you treat a frozen section is going to depend on what you are planning
to do with the frozen section. If you can be more specific that will help.
Do you want to immunohistochemical staining on a snap frozen tissue?
Do you want to fix and then store these frozen sections for
Do you want to store an unfixed frozen section for future
Do you want to do a rapid H&E for diagnostic purposes?
We do any one of the above depending on a particular staining procedure
At 11:04 AM 1/18/2007, you wrote:
>I have seen two basic camps regarding the treatment of frozen sections
>once the section hits the slide - One camp fixes immediately and one
>camp leaves the section to air dry, sometimes overnight. I realize that
>there is likely not a "right" answer, but are there advantages to one
>process method over another? I'm in the midst of trying out both
>processes and evaluating myself, but in the meantime I'd like to hear
>feedback from the community.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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