[Histonet] CD133 Help!!

[Makhijani, Nalini S]

I've never used this antibody- I was just suggesting a general technique
that I have seen work with overnight incubation rather than with a 1
hour
incubation, e.g.with Pecam 1.

-----Original Message-----
From: Tarango, Mark [mailto:mtarango <@t> nvcancer.org] 
Sent: Wednesday, January 17, 2007 10:45 AM
To: Makhijani, Nalini S; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

I tried that, but no luck.  Did you have some success incubating the
primary overnight?



Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

  

-----Original Message-----
From: Makhijani, Nalini S [mailto:Nalini.Makhijani <@t> va.gov] 
Sent: Wednesday, January 17, 2007 8:08 AM
To: Tarango, Mark; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

Try letting it go overnight.
Nalini Makhijani

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tarango,
Mark
Sent: Tuesday, January 16, 2007 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD133 Help!!

If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.

 

 

Thanks!!

 

 

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org <mailto:mtarango <@t> nvcancer.org> 

Direct Line (702) 822-5112

Fax (702) 939-7663

  

 


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