[Histonet] HSV ! and II

[Barry Madigan]

Sorry it has taken me so long to respond to this Post but time just seems to
fly.

We have been using a cocktail of HSV1 and HV2 for 8 years or more now here 
at the Royal Brisbane Hospital using both DAKO antibodies with excellent
results.
The current method is as follow:
A cocktail made of the following DAKO antibodies 
Polyclonal HSV 1	1:400 and DAKO Polyclonal HSV 2 1:800
No pre treatment.
Used on the BONDMAX Immunostainer. 
[15 minutes in Primary (no heat) with the VisionBiosystem Define Polymer
detection system]


Regards
Barry Madigan
Immunohistochemistry
QHPS- Central
Royal Brisbane Hospital
Australia


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Saturday, 6 January 2007 7:42 AM
To: Histonet
Subject: [Histonet] HSV

We have used combined HSV I & II from Biogenex. It has been on backorder and
now may be discontinued. We found an RUO from Biocare - which we want to
avoid. We can get I and II separately from DAKO and mix them. 
What are you all doing? Is there a combined non ASR or RUO somewhere? 
Have a good weekend and thanks for your help,
Joyce

Joyce Weems
Saint Josephs Hospital of Atlanta