[Histonet] HSV

[Richard Cartun]

Hi Joyce:

We been using an "HSV cocktail" for years with excellent results.  We
make a mixture of HSV-1 (B0114) and HSV-2 (B0116) from Dako at 1:1,000
each and incubate for 30' with Dako's EnVision+/AEC+ detection.  We do
not use antigen retrieval.    I'm not sure you really need HSV-2 since
HSV-1 will cross-react with HSV-II.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Weems, Joyce" <JWEEMS <@t> sjha.org> 01/05/07 4:42 PM >>>
We have used combined HSV I & II from Biogenex. It has been on
backorder and now may be discontinued. We found an RUO from Biocare -
which we want to avoid. We can get I and II separately from DAKO and mix
them. 
What are you all doing? Is there a combined non ASR or RUO somewhere? 
Have a good weekend and thanks for your help,
Joyce

Joyce Weems
Saint Josephs Hospital of Atlanta



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