[Histonet] immunocytochemistry

Melissa Mazan melissa.mazan <@t> tufts.edu
Fri Jan 12 14:01:25 CST 2007

Hi all, I haven't done immunofluorescence on cultured cells before, and 
one of my colleagues would like me to do some - he's grown up the cells 
on cover slips, then fixed with methanol/acetic acid.  They've been 
stored at room temp for the past week. Prior to staining, do I have to 
rehydrate them with graded alcohols? or just rinse with PBS or 
Thanks! Melissa

Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cumming School of Veterinary Medicine
200 Westborough Road
North Grafton, MA 01536
email: melissa.mazan <@t> tufts.edu

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