[Histonet] LUXOL FAST BLUE
twheelock <@t> mclean.harvard.edu
Fri Jan 12 12:54:41 CST 2007
I stain 5 micron paraffin sections of human brain with Luxol Fast Blue
Forget the Lithium.
The best differentiator is a 1% hydroquinone, 5% sodium sulfite aqueous
This will pull out the Luxol from everything except the white matter,
lipofuscin, and elastic fibers in arteries.
Works every time.
At least with my thin 5 micron sections, you only need 2 dips in the
Then immediately into 3 changes of tap water of about 20 dips each,
once more in tap water for 1 minute, and then a final holding dish with
Your 7 micron sections should behave the same. If you want to add 1 more
dip in the differentiator that should be fine too.
As for the pink stain that you have seen, it is probably Eosin.
I counter-stain the Luxol with:
(1) 10 minutes in Gill 3 Hematoxylin
(2) 3 minutes in Eosin Y Alcoholic.
So I end with a triple stain, an LHE (Luxol Fast Blue-Hematoxylin-Eosin)
The PAS should also work nicely once you differentiate the Luxol.
But as I hardly ever do this, I am not sure of the protocol.
However Sheehan's "Theory and Practice of Histotechnology" should have a
Hopes this helps.
I will send the entire protocol and an image to your email.
Harvard Brain Tissue Resource Center
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