[Histonet] Staining plant tissues

valleygal <@t> aol.com valleygal <@t> aol.com
Wed Jan 10 16:43:14 CST 2007

Depends on what you are staining for - are you looking for a good 
general stain? There are several protocols in the Ruzin book, Plant 
Microtechnique and Microscopy.

Andi Grantham

-----Original Message-----
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, 10 Jan 2007 3:33 PM
Subject: [Histonet] Staining plant tissues

Hello Histonetters,
I am looking for a good stain for plant tissue.  A couple of years ago 
tried Johansen's quadruple stain as described in Gray's Handbook of 
Microtechnique, but we were not satisfied with the results.  We fixed 
tissues in FAA (95% ethanol, glacial acetic acid, formalin).
Thank you in advance for any advice you can give me.

Elizabeth Wurdak, PhD
Biology Department
Saint John¹s University
Collegeville, MN 56321
Tel: (320) 363-3177

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